Welcome to Risun Bio-Tech Inc!

Tel:+86-29-8610-0730
Qeb khoom
Tiv tauj peb

TEL:+86-29-8610-0730

Fax:+86-29-8610-5620

E-mail: sale@risunextract.com

Web: www.risunextract.com

Xov xwm

Tsev > Xov xwm > Ntsiab lus
SAU LOS NTAWM PERILLA FRUTESCENS NPLOOJ EXTRACT RAU LIPOPOLYSACHARIDE-STIMULATED KAW264.7CELL
Risun Bio-Tech Inc | Updated: May 05, 2016

Sau los ntawm Perilla Extract of Lipolysacharide-stimulated RAW264.7cell


Perilla nplooj lug siv suav herbal tshuaj thiab Japanese herbal kab mob siv los kho tus kab mob ntsws. Txoj kev tshawb no tswj mus

soj ntsuam cov anti‑inflammatory los thiab tus mechanisms ntawm cov nplooj frutescens Perilla lwm extract (PLE). Murine macrophage RAW264.7 hlwb

yog siv los ua ib tug qauv. Nqi ntawm tes thiab morphological hloov tau kawm los ntawm MTT pom thiab microscopy. mRNA qhia txog

pro‑inflammatory neeg yog txheeb xyuas los ntawm semi‑quantitative transcription‑polymerase Confirmation chain reaction (RT‑PCR) thiab quantitative (q)

RT‑PCR. Nitric oxide (tsis muaj) thiab prostaglandin E2 (PGE2) ntau lawm tau analyzed ntawm tus Griess mus kuaj thiab nyuaj enzyme‑linked immunosorbent

pom (ELISA), feem. Qhov ua kom cov kinase cascades kawm los ntawm immunoblotting. Tshawb pom peb tsom tau tias PLE ib nyuag muaj feem xyuam rau ntawm tes

nqi, tab sis alleviates LPS‑induced ua kom cov hlwb RAW264.7. Tas, PLE nws txo cov LPS‑induced mRNA qhia txog qhov

(IL) interleukin ‑6, IL‑8, nqaij hlav necrosis factor‑α (TNF‑α), cyclooxygenase‑2 (COX‑2) thiab inducible nitric oxide synthase (iNOS), noob rau ib

cuj pwm Dose‑dependent. Tsis tas li, PLE txo tsis ntau lawm thiab PGE2 secretion vim raug yuam los ntawm LPS. PLE tseem inhibited ua kom cov

mitogen‑activated protein ntau kinases (MAPKs), si cytosolic IκBα qib, thiab txo cov qib uas tau nuclear (NF) ‑κB. Ua ke, noj no

tshawb pom qhia tias PLE yig decreases mRNA qhia thiab protein ntau zus tau tej cov pro‑inflammatory neeg, ntawm tus inhibition ntawm

extracellular‑signal‑regulated kinase (ERK) 1/2, c‑Jun N‑terminal kinase (JNK), p38, raws li NF‑κB signaling RAW264.7 hlwb stimulated LPS.